Hardware- and computation-based adaptive optical microscopy

Measurement and correction of optical aberrations in optical microscopy can be achieved through either hardware or computation. I will describe several recent projects from my laboratory where hardware including Shack-Hartmann wavefront sensors and segmented deformable mirrors, as well as a computational approach based on machine learning were used for aberration measurement and correction. These approaches were combined with several imaging modalities including widefield, confocal, and two-photon fluorescence microscopy for high-resolution imaging of in vivo structures.