Multimodal metabolic imaging of the ovary under diet manipulation during aging process

Lipid and protein metabolism play important roles in the aging process of ovary. High resolution in situ optical imaging provides a powerful approach to study the metabolic dynamics of macro-molecules such as lipids and proteins. Here, we applied a multimodal imaging platform integrated with D2O probing stimulated Raman scattering (DO-SRS), multiphoton fluorescence (MPF), and second harmonic generation (SHG) to explore metabolic changes of biomolecules in Drosophila ovaries during aging process. In this study, the sub-cellular spatial distribution of de novo protein synthesis, lipogenesis and redox ratio in ovaries are quantitatively imaged and examined in different ages. The regulation of diets on aging-dependant changes of ovary metabolism are investigated. Our results provide valuable insights for the underlying mechanism of ovary aging and how to intervene the aging process to achieve a better health