High-throughput and high-resolution single-cell biophysical fractometry for functional cell morphological profiling

We developed a large-scale morphological profiling approach that reads out a multitude of high-resolution biophysical fractal properties of single cells, based on our high-throughput quantitative phase imaging (QPI) platform (at 10,000 cells/sec). We showed that the single-cell morphological profile constructed by the sub-cellular fractal characteristics could be harnessed to implicate cell types and states in the context of identifying lung cancer cell line subtypes, assessing cell morphology in response to drug treatment, and tracking cell cycle progression.