Laser induced forward transfer of complex cell structures to study the interaction between tumor cells and lymph nodes in organs-on-chips

The study interactions between tumor cells and lymph nodes (LNs), will be investigated with the development of complex 3D cell structures in a microfluidic chip by Laser Induced Forward Transfer (LIFT) technique. The lymphatic system and LNs are an integral part of our adaptive immune system and many tumors exploit lymphatic vessels to spread and colonize downstream LNs. The complex cell structures will be realized by the printing of tumor organoids and the multilayer printing of 2D layers of LN cells, initially on ECM and other substrates, and ultimately inside a microfluidic chip.