Histomorphometric and Ultrastructural Study of Gestational Changes in Mouse Liver Using Haematoxylin and Eosin, Periodic acid Schiff stain

Abstract Background: Studying the makeup of a mouses liver, such, as its lobes, lobules and how hepatocytes are organized is important, for researching changes that occur during pregnancy. Aim of Study: the purpose of this study is to assess the structural, morphological, and general histological differences in liver tissue during pregnancy, including weight changes in the liver and total body weight, morphometric analysis and statistical assessment of hepatocytes, and glycogen concentration in the liver. Methodology: The study involved a comparative investigation between pregnant and non-pregnant mice on liver weight, total body weight, hepatocyte size, and glycogen concentration with utilized hematoxylin, eosin, and Periodic Acid Schiff stain for histological examination and applied statistical tools for data analysis. Results: Pregnant mice showed a significant increase (p=0.0001), in both liver and overall body weight and examination of the cell measurements indicated that the liver cells, in mice were larger indicating alterations. Glycogen concentration significantly increased in the livers of pregnant mice (p=0.0001), indicating altered metabolic demands. Conclusion: Pregnancy induces noticeable changes in liver weight, hepatocyte size, and glycogen storage, reflecting the liver's adaptive response to the increased metabolic needs during gestation.