Fast adaptive optics for deep imaging of biological media in light-sheet and two-photon microscopy

We report a fast, easy-to-use adaptive optics (AO) method and its implementation in both Light-Sheet and Two-Photon fluorescence microscopy setups. We demonstrate fast (typically 1 second), and accurate aberrations correction at large depths (hundreds of microns) mostly in brain tissue of animal models such as Drosophila, Zebra Fish and mouse. We quantifiy the gain brought by AO in terms of signal (up to x5), contrast, achievable imaging depth and segmentation capability of neuronal structures for both imaging modalities. The approach paves the way to future automated AO-based 3D microscopy systems.