Advanced biosensing method through fluorescence enhancement using photonic crystal fiber as optofluidic platform

We report on fluorescence enhancement using a suspended core photonic crystal fiber (PCF) as an optofluidic platform. By employing metallic nanoparticles and an organic spacer, we achieved a thirty-fold signal enhancement of Cy5 dye at picomolar concentrations. The combination of fluorescence enhancement and PCF offers robustness, ease of use, and high sensitivity. This comprehensive study explores fluorescence enhancement using PCF, highlighting the significant enhancement achieved through metallic nanoparticles and organic spacers associated by the long length of light-analyte interactions offered by the PCF. These findings might contribute to the development of highly sensitive optical fiber-platforms for biomedical applications.