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Cold atmospheric pressure plasma (CAP) has attracted increased interest in recent years for possible biomedical, environmental and agricultural applications. A wide range of cold plasma treatment effects is observed in agricultural applications, like effects on the seed germination and seedling growth, but more systematic investigations are needed. The aim of this study was to identify the most appropriate combinations of the plasma source and duration of treatment positively affecting seed germination. In addition, the effect of cold plasma on the seedling growth and osmotic stress tolerance was studied. The seeds of three Bulgarian durum wheat cultivars were treated with cold plasma in twelve variants. The results obtained were processed statistically via two-way ANOVA. The treatment of seeds with a plasma torch for 20 s and the treatment with underwater diaphragm discharge for 5 min when the seeds were placed in both cameras in two different positions (relative to the electrodes between which the plasma is supplied, “+” and “−”) have the greatest positive effect on the all traits related to germination. The analysis of variance reveals that the variation in germination energy, shoot length and root length after the cold plasma treatment of seeds is mainly due to the interaction between the genotype and treatment variant and to a small degree due to the genotype. The treatment of seeds with cold plasma improves the osmoregulation ability of cells and therefore increases the drought resistance of genotypes.
Bozhanova et al. (Sun,) studied this question.