The purpose of this experiment is to investigate the effects of forskolin-mediated cAMP activation on NF-κB, TNF-ɑ, AKAP95, and cyclin D3 expression in LPS-treated Schwann cells. The immortalized rat RT4-D6P2T cell line (ATCC #CRL-2768) was cultured and received one of the following treatments: 0.1, 1, or 10 μg/mL of LPS, in N2 media (control) or N2 media supplemented with 2 μM of forskolin, for 3 hours. Cell lysates were prepared, and immunoblotting was subsequently performed to quantify changes in NF-κB, TNF-ɑ, AKAP95, and cyclin D3 expression in response to the different treatment combinations.
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C Henry (2024) studied this question.
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