What question did this study set out to answer?

Investigate the intracellular signaling pathways utilized by tumor necrosis factor (TNF) and interleukin 1 (IL-1) in human fibroblasts, focusing on cyclic AMP production, protein kinase activation, and interleukin 6 (IL-6) gene expression.

September 1, 1988Proceedings of the National Academy of SciencesOpen Access

Enhancement of cAMP levels and of protein kinase activity by tumor necrosis factor and interleukin 1 in human fibroblasts: role in the induction of interleukin 6.

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Authors

YZY H ZhangNew York University JLJ X LinNanchang University YYY K YipCity University of New York

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Implication

In vitro study reveals that TNF and IL-1 trigger cAMP signaling and protein kinase activation in human fibroblasts, indicating a multifaceted mechanism for interleukin 6 induction.

Key Points

Investigate the intracellular signaling pathways utilized by tumor necrosis factor (TNF) and interleukin 1 (IL-1) in human fibroblasts, focusing on cyclic AMP production, protein kinase activation, and interleukin 6 (IL-6) gene expression.
Treated human FS-4 fibroblasts with TNF, IL-1, or forskolin, both in the presence and absence of the protein kinase inhibitor H-8.
Assayed intracellular cAMP kinetics, protein kinase C activation, histone HII-B phosphorylation, and IL-6 mRNA expression levels.
Intracellular cAMP levels peaked rapidly within 3–5 minutes after exposure to TNF or IL-1 and returned to baseline by 15 minutes, accompanied by increased phosphorylation of histone HII-B without protein kinase C activation.
TNF, IL-1, and forskolin each induced IL-6 mRNA accumulation, but the protein kinase inhibitor H-8 suppressed forskolin-induced expression substantially more than TNF- or IL-1-induced expression.

Cite This Study

Zhang et al. (1988) studied this question.

synapsesocial.com/papers/6a0deaab6e03bc61cb09fb0e https://doi.org/10.1073/pnas.85.18.6802

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Implication

In vitro study reveals that TNF and IL-1 trigger cAMP signaling and protein kinase activation in human fibroblasts, indicating a multifaceted mechanism for interleukin 6 induction.

Enhancement of cAMP levels and of protein kinase activity by tumor necrosis factor and interleukin 1 in human fibroblasts: role in the induction of interleukin 6.

Discussion

Member takes

Implication

Key Points

Cite This Study

Enhancement of cAMP levels and of protein kinase activity by tumor necrosis factor and interleukin 1 in human fibroblasts: role in the induction of interleukin 6.

Discussion

Member takes

Implication

Key Points

Cite This Study

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