Natural Phenolics Exhibit Strong Antimicrobial activity against Clinical Isolates Obtained from Patients of Urinary Tract Infection

A Comprehensive study of the Antimicrobial efficacy of Gallic acid and Tannic acid on the clinical isolates of the UTI microflora as a promising component. This was a Cross sectional study carried out in the Department of Microbiology at Om Sterling Global University, Hisar, Haryana, India for a period of 1 year i.e, between June 2022 to June 2023 upon pathogenic bacteria from urine samples of UTI patients visiting the New Reliable Path labs, Qurban Road, Chandwara, Muzaffarpur (Bihar), India. A total of 350 freshly voided midstream urine samples from the people whose initial routine urine tests were positive for pus cells and albumin were collected in a sterile wide mouth container. The Gallic acid (CAS No 5995-86-8, product code 27645) and tannic acid (CAS No. 1401-55-4, product code 403040) standards were purchased from Merck, St. Louis, MO, USA where the stock solutions of both gallic acid and tannic acid were prepared by dissolving 50 mg of the compound in 1 mL of phosphate-buffered saline (PBS) containing 10% DMSO, to determine the minimum inhibitory concentration. The MICs and MBCs were determined performing broth dilution. In the present study it was observed that the tannic acid (T.A.) was more effective as compared to the gallic acid (G.A.). The MIC value of 225±1.06 was observed for T.A. and 1750±18.66 for G.A. against E coli. The lowest MIC of tannic acid was observed for Staphylococcus saprophyticus 90±1.06 followed by Acinetobacter baumannii with 125±3.14 and highest for Proteus vulgaris375±6.14. In case of gallic acid the lowest MIC was recorded for Pseudomonas aeruginosa with 600±8.66 followed by citrobacter freundii with 750 ±3.14 and the maximum was observed for Klebsiella pneumoniae observed 2050 ±1.21. Tannic acid can serve as a promising candidate for treating (multi drug resistant) MDR Urinary Tract Infections. It was also found that the drugs with lower MIC scores are more effective antimicrobial agents.