FIGURE 5 from Gigaxonin Suppresses Epithelial-to-Mesenchymal Transition of Human Cancer Through Downregulation of Snail

Gigaxonin re-expression leads to reversal of EMT phenotype. A, Incucyte growth assay shows gigaxonin re-expressing cells with statistically significant (P < 0.001) slower growth than control lentiviral transfected cells. Differential growth between parental GAN edited cells and that of control lentiviral transfected cells is not statistically significant (P = 0.1088). B and C, Migration assay shows minimal migration of ME180 cells. There is statistically significant (P < 0.0001) increased migration of GAN edited and control viral transfected cells which is reduced with the re-expression of gigaxonin. D and E, Invasion through Matrigel again shows absence of invasion in ME180 cells that dramatically increases in GAN edited cells. Gigaxonin re-expressing cells show statistically significant (P < 0.0013) reduced Matrigel invasion in comparison with the control viral transfected cells.