FIGURE 4 from Gigaxonin Suppresses Epithelial-to-Mesenchymal Transition of Human Cancer Through Downregulation of Snail

Re-expression of gigaxonin leads to reversal of NFκB pathway. A, Re-expression of gigaxonin in GAN edited cells showed an intermediate epithelial morphology with a brighter light transmission than GAN edited cells or control lentiviral clones. B, Circos plots of the exome sequence analysis showing similar peaks for SNP in chromosomes 9 and 11 and intrachromosomal deletions (indels) for chromosomes 7, 15, and 16 in the parental ME180, GAN edited, and the lentiviral transfected clones. C, Re-expression of gigaxonin leads to increased expression of epithelial marker e-cadherin. Loss of n-cadherin and Snail expression are seen in gigaxonin re-expressing cells confirming inverse relationship between the gigaxonin and EMT markers. D, GO program of the RNA-seq data shows reversal to myogenesis signaling, and TNFα signaling via NFκB pathways in GAN re-expressing ME180/C4.15.1/LV GAN 50 cells. Lentiviral aliquots 25, 50, and 250 represent transfection of 5 × 10³, 1 × 10⁴, and 5 × 10⁴ viral particles.