FIGURE 2 from Gigaxonin Suppresses Epithelial-to-Mesenchymal Transition of Human Cancer Through Downregulation of Snail

Inverse relationship between gigaxonin expression and cancer cell line growth in HPV18 and HPV18- versus HPV16-positive cell lines. A, Statistically significant reduced in vitro cell growth is seen in ME180 in comparison with HeLa cells. B, Soft agar colony formation assay confirms reduced growth of ME180 cells. Although HeLa and SiHa cell lines contain C/T alleles, HeLa cells with higher gigaxonin expression than SiHa cells. C, Shows in vitro slower growth. D, Reduced soft agar colony formation and increased cisplatin sensitivity. E, Incucyte growth assay demonstrates increasing sensitivity of ME180 cells with increasing concentration of cisplatin. Cell images acquired during the Incucyte assay shows presence of live cells 72 hours after cisplatin treatment (Supplementary Fig. S6). F, MTT assay demonstrates presence of live cells 6 days after cisplatin treatment (30%, 10%, and 6% live cells compared with untreated control in 0.5, 1.0, and 3.0 µg/mL of cisplatin treatment, respectively). G, Cells collected after 4 days of cisplatin treatment show decreased expression of GAN, and p16 (CDKN2A) and enhanced NFκB expression. The data suggest that NFκB activation is related to the growth of cisplatin-resistant cells.