March 7, 2024Scientific ReportsOpen Access

Optimization of induction and hairy root culture establishment in two mullein species, Verbascum erianthum and Verbascum stachydiforme

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Authors

SASoniya AminiUrmia University MFMohammad FattahiUrmia University HNHossein NazemiyehTabriz University of Medical Sciences

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Implication

Laboratory study reveals optimal induction and transformation parameters for hairy roots in two mullein species, highlighting scalable secondary metabolite production.

Key Points

Hairy root culture was successfully established in Verbascum erianthum and Verbascum stachydiforme, yielding substantial secondary metabolites including flavonoids.
A transformation efficiency of 80.55% was achieved using Agrobacterium rhizogenes strain A13 in 21-day-old leaf explants, with PCR confirming rolb gene integration.
Experimental evaluation across explants and media additives suppressed tissue browning to 9%, which may enable efficient production of bioactive iridoid glycosides.

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Amini et al. (2024) studied this question.

synapsesocial.com/papers/68e7542bb6db6435876cc054 https://doi.org/10.1038/s41598-024-56331-8

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Implication

Laboratory study reveals optimal induction and transformation parameters for hairy roots in two mullein species, highlighting scalable secondary metabolite production.

Optimization of induction and hairy root culture establishment in two mullein species, Verbascum erianthum and Verbascum stachydiforme

Discussion

Member takes

Implication

Key Points

Cite This Study

Optimization of induction and hairy root culture establishment in two mullein species, Verbascum erianthum and Verbascum stachydiforme

Discussion

Member takes

Implication

Key Points

Cite This Study

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