Yeast gDNA isolation v1

This protocol is based on https://cshprotocols.cshlp.org/content/2020/10/pdb.prot098152.full doi:10.1101/pdb.prot098152 The protocol details how to perform a small scale yeast gDNA isolation including a proteinase K incubation step to visualize linear cytoplasmic protein primed plasmids. This is used in combination with the system OrthoRep which relies on error prone replication of linear cytoplasmic plasmids. Rix, G., Watkins-Dulaney, E.J., Almhjell, P.J.et al.Scalable continuous evolution for the generation of diverse enzyme variants encompassing promiscuous activities.Nat Commun11, 5644 (2020). https://doi.org/10.1038/s41467-020-19539-6