What question did this study set out to answer?

This research aims to develop a rapid and effective protocol for the extraction of small chromosomes and plasmids from various yeast species.

January 23, 2026Synthetic BiologyOpen Access

EASY-C: extraction and analysis of small yeast chromosomes—a rapid and universal platform for recovering artificial mini-chromosomes from synthetic Sc2.0 yeast and large plasmids from S. Cerevisiae and non-conventional yeast species

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EASY-C demonstrates efficient isolation of synthetic chromosomes in yeast, indicating a powerful tool for genetic validation.

This research aims to develop a rapid and effective protocol for the extraction of small chromosomes and plasmids from various yeast species.
Developed a two-step protocol for DNA extraction and recovery.
Extracted DNA from Saccharomyces cerevisiae and non-conventional yeast species.
Performed downstream analysis including long-read sequencing.
Utilized standard plasmid purification kits for extraction.
Achieved a yield of clean DNA suitable for digestion and sequencing.
Produced around 2000 high-quality reads.
Success observed across multiple constructs, including low-copy centromeric vectors and high-copy plasmids.

Swidah et al. (2026) studied this question.

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