combined sequence dataset verified the identification.To confirm the pathogenicity, the roots of five apple seedlings were wounded and dipped in 50 ml of the conidia suspension of 1 × 10 6 spores/ml from a 15-day-old culture.The inoculated seedlings were cultivated in a growth chamber at 25 °C.Controls were inoculated with sterile distilled water.After five weeks, the pathogen caused brown to red discolored areas on crown and root of apple seedlings.The pathogen was re-isolated and re-identified.No symptoms were observed in the control.To our knowledge, this is the first report of apple crown and root rot caused by F. oxysporum in Turkey.
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Ören et al. (2024) studied this question.
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