Abstract 1941 The Use of Confocal Imaging To Determine Structural And Cytotoxic Effects of An Acridine Derivative in Lung Cells

In our pursuit of effective anticancer solutions, we chose to focus on acridine compounds, which have an established record of high cytotoxic activity and potential anti-cancer properties. In this study we investigated the anticancer properties of an acridine compound with a modified nitrogen heterocyclic ring in A549 lung cancer cells. These cells are a part of the NCI60 panel, and have been selected for their recognized importance in cancer research. This trial used confocal imaging to examine the changes induced by the acridine compound in comparison to untreated control cells. A549 cells were dosed with 0.25μM of compound, and subsequent nuclear changes were monitored through confocal microscopy. Our study identified significant changes, including distinct alterations in cellular and nuclear morphology upon treatment with our compound compared to untreated controls. Notably, the treated cells exhibited an increase in nuclear size and noticeable changes in cell shapes, providing evidence of the compound's impact on cellular structures. Furthermore, our study revealed a decrease in cell viability in the treated group, supporting the cytotoxic effects of the compound. This study contributes to the growing body of knowledge on acridine-based compounds in cancer research. These molecular and cellular changes lays the foundation for future investigations into the therapeutic potential of the acridine compound and opens avenues for the development of targeted cancer therapies. Funding for this project has come from Georgia Southern Department of Biochemistry, Chemistry, and Physics, GS FRC SEED GRANT (FY21) and NIH (Grant Award #1R15CA277652).