Abstract 1401 Metabolomic Analysis using Charged Cyclodextrins in an Electrochemical Biosensor

Cyclodextrins are cyclic oligosaccharides composed of 6, 7, or 8 glucose subunits, and are especially promising for use in sensors due to their hydrophilic surface and hydrophobic inner cavity [1, 2]. Cyclodextrins can form inclusion complexes or hydrogen bond with different chemicals. The SEEDS Lab was successful in developing a biosensor to detect cortisol by grafting diazonium-salt to a carbon surface followed by attachment of polypropylene glycol [3]. Cyclodextrin was then loaded onto the surface and released when interacting with cortisol at low concentrations [2, 3]. Electrochemical Impedance Spectroscopy (EIS) serves as a powerful technique to monitor the response of our CD surface. In this recent work, we will present the effect of using negatively charged sulfobutylether-βCD and positively charged monoamine-βCD for the detection of creatinine, ribonic acid, and erythronic acid. By using charged cyclodextrins, we aim to target charged analytes for greater metabolomic analysis towards disease diagnosis. Initial findings suggest that incorporating charged cyclodextrins enhances surface sensitivity. In addition to our initial findings, our progress on refining our electrochemical techniques towards metabolomic analysis using cyclodextrin will be presented. References: [1] S.S. Jambhekar and P. Breen. Drug Discovery Today. 21 (2016) 356-362. [2] Z. Panahi, et al. ACS Applied Polymer Materials. 2 (2020) 5086-5093. [3] Z. Panahi, et al. ACS Applied Materials & Interfaces. 14 (2022) 42374- 42387. Research supported by New Hampshire- INBRE through an Institutional Development Award (IDeA), P20GM103506, from the National Institute of General Medical Sciences of the NIH and the Hamel Center for Undergraduate Research at the University of New Hampshire.