Abstract 1676 Comparative Analysis of Differential Gene Expression of Cultured Cell Lines Exposed to Bisphenol-A and Taxol

In previous studies, Bisphenol-A (BPA) has been shown to have adverse effects on cell division in mammalian cells. In the presence of BPA, tubulins displayed disruptions in the spindle apparatus during cell division in mitosis and meiosis. Like Taxol or Colchicine, BPA may have the ability to bind directly to free tubulin subunits; and impairs the formation of normal polymers that make up spindle fibers. Therefore, BPA poses a threat to cell division in mammalian cells due to its capacity to disrupt chromosome segregation like Taxol or Colchicine. This study intends to analyze and characterize spindle assembly genes and cytoskeletal proteins that are expressed in cancerous and non-cancerous cells when exposed to BPA compared to other known tubulin disruptors, like Taxol. There will also be focused attention on whether the expression of these genes could potentially lead to possible targets for cancer therapy. We intend to conduct this study by growing cell cultures, HEK-293 (non-cancerous) and MCF7 (cancerous) cell lines, in both control and experimental groups. We will arrest all samples at the G1/S boundary of the cell cycle by double thymidine block to synchronize the cell cultures. After cells are released from the thymidine block and exposed to BPA or Taxol during the M phase. We will then collect samples as the cells progress through mitosis. Upon collection of the samples, we will isolate RNA transcripts by RNA isolation protocol and create cDNA in preparation for RNA seq analysis. Post RNA isolation, we will send the cDNA samples to the Cancer Center Genomics Shared Resource Facility to provide gene sequences. Lastly, we will analyze and compare the gene sequences by creating genetic profiles of what specific genes are expressed when cancerous and non-cancerous cells are exposed to BPA and Taxol. The application of the information obtained by this study will allow other researchers to further understand how the mitotic expression of genes or cytoskeletal proteins is important when treating cancer cells, and determine whether or not these specific genes are possible targets for cancer therapy in future studies. This project was supported by grants from the National Institutes of Health (NIH), National Institute of General Medical Sciences (NIGMS), IDeA Networks of Biomedical Research Excellence (INBRE), Award number: . The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.