Evaluation of Gelatin-PCL scaffold using polynomial model for skin tissue engineering

Tissue engineering research is widespread today due to the need to use skin tissue in patients who have lost skin from burns and accidents.When the skin is damaged so large and deep that the body cannot repair itself.Skin replacement tissue is therefore a part in helping the skin recover.However, this skin replacement tissue is still costly [1,2].Producing skin replacement tissue with its properties similar to real skin was challenging due to the complex structure of skin, such as flexibility, breathability, and softness.Nowadays, researchers have devised ways to produce artificial or synthetic dermatitis and cultured cells that can be biodegradable to transplant the wound area.*Author for correspondence Thus, it becomes possible to repair damaged skin by using skin substitutes, known as "scaffold".Scaffold is collagen, a widely used natural polymer.It is used in tissue repair and regeneration, which has biological compatibility and can be mixed with other synthetic polymers to help increase the scaffold's properties.Because in previous research, it was found that the pure gelatin scaffold was lack of the strength properties comparable to real skin.Therefore, there was a need to add synthetic polymers into the mixture to enhance the desired properties of the synthetic skin.Commonly used biodegradable polymers for blending such as carboxymethyl cellulose (CMC), agarose etc [3].In this research, skin substitutes containing gelatin and gelatin/polycaprolactone (PCL) were studied in various ratios in order to have properties similar to real skin.The forming process