Determination of Non-Cytotoxic Concentrations of Purine Analogues on Different Types of In Vitro-Incubated Embryonic Cells: A Pilot Study

Different concentrations of the methylxanthine/purine analogues aminophylline and 61-tartrat, were tested on in vitro-incubated embryonic avian cells from duck line DEC 99, as well as of mammalian embryonic cells from bovine line EBTr and mouse fibroblast line 3T3. In all cases, the C\(C₅₀\)/ml cell suspension, presenting the cytotoxic concentration, in which were observed 50% death or changes, was determined. On the avian embryonic cells, the determined values of the methylxantine/purine analogues were 2.4x \(10⁻⁶\) M/L about the aminophylline and 2.1x \(10⁻⁶\) M/L about the 61-tartrat. The assessed C\(C₅₀\)/mL of the same compounds on the mammalian cells were 2.4x \(10⁻⁵\) M/L about the aminophylline and 2.1x \(10⁻⁵\) M/L about the 61-tartrat, respectively. The embryonic mammalian cells were more resistant to both substances than the embryonic avian cells. On the other hand, the assessed values of both C\(C₅₀\)/ml and maximal non-toxic concentration (MNC - in which no cellular mortality or other changes can be detected) of each one of the two methylxantine/purine analogues were very near about EBTr and 3T3 mammalian cell lines, and the mouse cells, which are proved as widely used experimental in vitro-model, showed some advantages in both cases. In this way, the current data suggest a possibility about application of methylxantine/purine derivatives about genetic and/or epigenetic reparations, on DNA- and mRNA-levels of low differentiated mammalian cells.