More Evidences for the Possible Role of Err Beta (ERRβ) as a Tumor Suppressor in Estrogen Receptor Positive and Negative Breast Cancer

Estrogen-related receptors (ERRs) are a group of nuclear receptors that share sequence homology with ER whereas do not bind to Estrogen.In this article, studies were conducted with ERRβ, which is considered to have antiproliferative capabilities, to summarize its effects upon overexpression and knockdown.Clonogenic assay, and vivo zebra fish model tumour xenograft assay was used to detect the tumour growth and suppression.Wound healing and trans well migration as well as invasion assays were used to detect the metastasis in breast cancer.In this study, we established ERRβ as a possible tumour suppressor.Furthermore, epithelial-mesenchymal transition (EMT) pathway was checked with respective markers and it was observed that ERRβ overexpression in MCF7 and MDAMB -231 cells reduced the expression of mesenchymal markers where in promoting an upregulation of epithelial markers.Using triple negative MDAMB 231 cell lines, the zebra fish model was also utilized to check for tumor growth and migration.It was discovered that overexpression of the ERRβ resulted in a considerable reduction in the size of the tumor and its metastasis.Overall, our research showed that ERRβ interferes with breast cancer cell ability to proliferate and metastasize.Therefore, emphasizing on the function of ERRβ and, in turn, the process of EMT, may prevent the oncogenesis and metastasis in breast cancer cells.