FIGURE 3 from Characterizing Neutrophil Subtypes in Cancer Using scRNA Sequencing Demonstrates the Importance of IL1β/CXCR2 Axis in Generation of Metastasis-specific Neutrophils

CD4⁺ T cells are transcriptomically altered in metastasis. A and B, UMAP plots of CD4⁺ and CD8⁺ T cells in PT and LM grouped by cell type and tumor type, respectively. C, Differential gene expression of CD4⁺ T cells in LM compared with PT. D and E, GO and KEGG analyses of metastatic CD4⁺ T cells. F and G, Global cell-cell communication network and the interaction strengths between neutrophils, CD4⁺, CD8⁺, and Tregs. H–J, The contribution of each L-R pair to the overall signaling pathway for CXCL, IL1, and TNF pathways. K–M, Visualization of the cell-cell communication patterns mediated by each significant L-R pair for the three pathways.