Multiplex Real-Time PCR Assessment of Semen Quality and Male Fertility Impacted by Sexually Transmitted Infections

Introduction : Sexually transmitted diseases (STDs) are a severe problem because many infections are asymptomatic or have fewer symptoms and account for about 20% of male infertility. Objective: This study developed Real-time PCR kits to detect Treponema pallidum , Neisseria gonorrhoeae , Mycoplasma species, and Trichomonas vaginalis in infertile and fertile men's semen. Methods : Men aged 24–55 with no children and a conceiving issue were the patient group, while men aged 18–49 with at least one child and a standard seminal fluid check were the control group. The Amplisens® MULTIPRIME-FRT PCR kit was used to analyze the DNA for the human beta-globin HBB gene to determine if it came from the isolated pathogen. Results: The study revealed that out of the patient group, 22 out of 54 individuals tested positive (40.9%) for N. gonorrhoeae , 7 out of 54 (31.8%) for T. pallidum , 4 out of 54 (18.18%) for M. genitalium , and 2 out of 54 (9.09%) for T. vaginalis . In the control group, which consisted of 13 males, only one infection with N. gonorrhoeae was detected. Conclusion : The four urogenital infections evaluated were common in infertile people. However, they may not be the only cause of male infertility in this region.