Development of a Laboratory Stand for Automated Mechanical Production of Stromal-Vascular Fraction From Adipose Tissue

Adipose tissue, consisting primarily of adipocytes organized within lobules and surrounded by a stromal matrix, has gained attention for its potential therapeutic applications, particularly in regenerative medicine. Stromal-vascular fraction (SVF), a heterogeneous mixture of cells including mesenchymal stem cells, endothelial cells, and macrophages, has shown promise in various clinical settings. While enzymatic methods have been traditionally employed for SVF isolation, mechanical processing offers an alternative with its own set of advantages and challenges. This study focuses on the development of a laboratory stand for the automated mechanical production of SVF with controlled impact, aiming to provide a standardized platform for research and potential clinical applications. The designed system integrates a linear motion unit, control unit, and personal computer for precise control and data collection. By utilizing force sensors and Hagen-Poiseuille equations, the viscosity of the medium between syringes can be estimated in real-time, facilitating the optimization of mechanical processing parameters. Experimental validation using model media with viscosities akin to lipoaspirate demonstrates the feasibility and efficacy of the laboratory stand. Further studies are planned to assess SVF cell survival using different types of connectors, paving the way for enhanced understanding and optimization of mechanical SVF isolation techniques.