Development and validation of an UPLC–MS/MS assay for the simultaneous quantification of seven commonly used antibiotics in human plasma and its application in therapeutic drug monitoring

Xin Meng Mekking; Kirsten Velthoven‐Graafland; Marga Teulen; Roger J. M. Brüggemann; Lindsey H. M. te Brake; Nynke G L Jager

doi:10.1093/jac/dkae047

February 28, 2024Journal of Antimicrobial ChemotherapyOpen Access

Development and validation of an UPLC–MS/MS assay for the simultaneous quantification of seven commonly used antibiotics in human plasma and its application in therapeutic drug monitoring

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Key Points

Simultaneous quantification of seven plasma antibiotics alongside unbound flucloxacillin was successfully established through a validated UPLC-MS/MS bioanalytical method.
UPLC-MS/MS assay development enabled concurrent determination of ceftazidime, ciprofloxacin, piperacillin, and tazobactam total concentrations in human plasma matrices.
Therapeutic drug monitoring protocols gain an efficient quantitative platform for multiple antimicrobial agents, supporting optimized dosing regimens in clinical practice.

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Implication

Assay validation study demonstrates simultaneous quantification of seven antibiotics in human plasma, highlighting utility for therapeutic drug monitoring in clinical practice.

Abstract

To develop and validate an UPLC-MS/MS assay for simultaneous determination of the total concentration of ceftazidime, ciprofloxacin, flucloxacillin, piperacillin, tazobactam, sulfamethoxazole, N-acetyl sulfamethoxazole and trimethoprim, and the protein-unbound concentration of flucloxacillin, in human plasma to be used for research and clinical practice.

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Mekking et al. (2024) studied this question.

synapsesocial.com/papers/68e77226b6db6435876e7b19 https://doi.org/10.1093/jac/dkae047

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Development and validation of an UPLC–MS/MS assay for the simultaneous quantification of seven commonly used antibiotics in human plasma and its application in therapeutic drug monitoring

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Development and validation of an UPLC–MS/MS assay for the simultaneous quantification of seven commonly used antibiotics in human plasma and its application in therapeutic drug monitoring

Key Points

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Implication

Abstract

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