Study on the Antagonistic Activity Against Two Pathogenic Bacteria in Humans of Streptomyces Mip_l26 Isolated From Soil in the Root Zone of the May Chang Tree (Litsea Cubeb)

Purpose: Study on the antagonistic activity against two pathogenic bacteria in humans (Staphylococcus aureus and Streptococcus pneumoniae) of the Streptomyces MIP_L26 (Called MIP_L26), isolated from the soil in the root zone of the May Chang tree; to investigate the biological characteristics of MIP_L26 Subjects and methods: Experimental study, determine the antimicrobial activity against two pathogenic bacteria in humans (Staphylococcus aureus and Streptococcus pneumoniae), and some biological characteristics of MIP_L26. Results: MIP_L26 was identified as having a strong antagonistic ability against the two bacteria causing Staphylococcus aureus and Streptococcus pneumoniae with an antibacterial ring diameter of 18 ± 5 mm and 13 ± 5 mm, respectively. Based on classification according to morphological, physiological, and biochemical characteristics, combined with the results of 16S rRNA gene sequencing, MIP_L26 was identified as Streptomyces gancidicus MIP_L26. The fermentation medium for the biosynthesis of antibacterial substances was ISP6 and the recovery time for antimicrobial substances was after 72 hours of fermentation.