FIGURE 7 from INHBA is Enriched in HPV-negative Oropharyngeal Squamous Cell Carcinoma and Promotes Cancer Progression

Colony formation ability upon INHBA knockdown. A, Ability for clonal expansion of native UDSCC1 cells, Mock KD, INHBA KD1/KD2, and UDSCC2 cells as assessed by colony formation assay. Colonies between 10 and 50 cells were considered small (red) and colonies greater 50 cells were considered big (blue). Results are shown as mean ± SEM of the number of total colonies (n = 3). Conditions were compared with native UDSCC1 using unpaired t test with **, P ≤ 0.01; ***, P ≤ 0.001; and ns, P > 0.05. B, Representative images of small and big colonies in UDSCC1 and UDSCC2 cells stained with crystal violet. C, Representative images of colony formation for each condition.