Lonicerin Alleviates Interleukin-1β-Stimulated Extracellular Cartilage Matrix Degradation and Inflammation

This study aimed to investigate the effects of lonicerin on an osteoarthritis model in vitro and determine its mechanism of action. We induced SW1353 cells with interleukin-1β to construct a cell model of osteoarthritis. The effects of lonicerin on cell viability were observed using cell counting kit-8 assays. Quantitative polymerase chain reaction, immunoblot, and enzyme-linked immune-sorbent assays revealed the effects of lonicerin on inflammation. Additionally, quantitative polymerase chain reaction and immunoblot assays showed the degree of extracellular matrix degradation. Immunoblot assays were further performed to confirm the mechanism of action of lonicerin. Lonicerin contributed to interleukin-1β-stimulated SW1353 cell viability. Furthermore, lonicerin restrained the inflammatory response of interleukin-1β-stimulated SW1353 cells, suppressed the extracellular matrix degradation of interleukin-1β-stimulated SW1353 cells, and increased collagen II expression. Mechanically, lonicerin suppressed the nuclear factor-kappa B pathway and prevented osteoarthritis progression. Lonicerin alleviates interleukin-1β-stimulated cartilage extracellular matrix and inflammation.