Key points are not available for this paper at this time.
In this study, a pH-response polyacrylate amphiphilic copolymer PMDB was used as the carrier to immobilize laccase from trametes versicolor. Under optimized conditions, the activity recovery of the immobilized laccase is 71.90%. The infrared spectroscopy results indicate laccase is successfully immobilized on the polymer PMDB. Also the properties of the immobilized laccase were studied. The recovery of the immobilized laccase is 96.8% (wt%) by adjusting the pH of the immobilized laccase solution to 3.3. And the immobilized laccase remains 78.13% of the initial activity after 5 batches reactions. Compare to the free laccase, the stabilities like thermal and storage stabilities of the immobilized laccase are enhanced. Moreover, the relative activity of immobilized laccase is nearly 110% when the concentration of urea in the solution is 0.02 M. The work in this manuscript can provide valuable references for enzyme immobilization.
Yu et al. (Fri,) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: