FIGURE 5 from Stromal Signals Dominate Gene Expression Signature Scores That Aim to Describe Cancer Cell–intrinsic Stemness or Mesenchymality Characteristics

RosettaSX analysis of an integrated dataset of TCGA colorectal cancer (CRC) and fibroblast-enriched pseudobulk samples. Top annotation indicates the tumor purity (CPE) for TCGA samples, and the fraction of fibroblast cell RNA used for pseudobulk generation (see Materials and Methods). The tumor samples (columns) are sorted by their tumor purity from low (left) to high (right). Note the tight clustering of all 11 EMT-related signatures and their correlation with tumor purity.