Mime-seq 2.0: a method to sequence microRNAs from specific mouse cell types v1

The description of precise miRNA expression patterns is crucial to understand what these small RNAs contribute to animal development and physiology. High-throughput sequencing of miRNAs from individual developmental stageshasprovided insightinto temporal regulationbut often lacks thecellularresolution to link miRNA-function to the biology of distinct cell types within complex tissues.Here, weprovide a protocol formicroRNomeby methylation-dependent sequencingfor mice(mime-seq 2.0).Mime-seq takes advantage ofa chemical small RNA-tagging approach followed bychemo-selective, high-throughput sequencing that enables the identification of tissue- and cell type-specific miRNA profiles in animals in a sensitive and reproducible manner.