FIGURE 5 from Estrogen Receptor Mutations as Novel Targets for Immunotherapy in Metastatic Estrogen Receptor–positive Breast Cancer

Peptide-specific CTLs effectively kill ER⁺ breast tumor cells. MCF7 cells were pulsed with corresponding peptides and labeled with calcein-AM. Cytotoxicity was determined by a standard calcein-AM release assay. MCF7 cells were pulsed with corresponding peptides. CG1-pulsed MCF7 cells and non-pulsed MCF7 cells were used as negative controls. Statistical significance was determined via one-way ANOVA. Data show percent cytotoxicity at the 10:1 effector:target ratio, and represent the average cytotoxicity of five experiments (run in triplicate) from different healthy female donors. *, P < 0.05; ***, P < 0.001.