In vitro Cytotoxicity and Apoptotic Activity of Aquous, Chloroform and Methanol Extracts of Kiwi Fruit Actinidia deliciosa in MCF-7 cells

The study aimed to assess the anticancer potential of Aquous (AQU), Chloroform (CHL) and Methanol (METH) extract of Actinidia deliciosa on the MCF-7 cell line. MTT cytotoxicity assay was used to determine the anticancer activity of the fruit extracts. DAPI was also used to determine the apoptotic cell imaging. The MTT assays revealed dose-dependent cytotoxic effects, with METH extract exhibiting the highest potency, with an IC50 value of 70.984 μg/ml after 24 hours of incubation. DAPI staining confirmed apoptotic cell death induced by the extract. The observed cytotoxic and anticancer effects are likely attributed to bioactive compounds in A. deliciosa fruit extracts. These findings suggest the potential of A. deliciosa METH extract as a natural source for cancer medication development. Further investigation is needed to identify the active compounds responsible for these effects and their apoptotic pathways. Future research should explore its efficacy in vivo and its potential as a dietary supplement or therapeutic agent.