Making Agarose for use in acute in vivo Electrophysiology Experiments v1

This protocol describes the procedure for making agarose used in acute in vivo Electrophysiology Experiments with Neuropixels probes. This exact methodology is crucial to the the success of these types of experiments due to the requirements that the agarose must be strong enough to provide the brain stability while also being penetrable by the delicate probes. This agarose preparation protocol is used in Neuropixels Data Collection: Whole Hemisphere Recordings and Visual Cortex Recording Window Replacement for in vivo Elctrophysiology Experiments.