An Updated Stepped Elongation Time (STeP-Up) Sanger Sequencing Protocol to Economize Workflows and Increase Useability

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Sanger sequencing methods are an important contribution to the field of genomics. DNA sequencing has a multitude of applications including medical diagnostics, identifying mutations and validating NGS results. Standard DNA sequencing reactions can be time consuming and expensive; these factors may limit the potential of its applications on a larger scale. This updated Stepped Time Elongation (STeP) protocol aims to increase cost effectiveness and DNA sequencing efficiency, while maintaining the highest quality results as standard DNA sequencing.