The Beneficial Effect of Lactiplantibacillus Plantarum DM083 on Restoring the Hyperglycemia in High-Sucrose Diet-Fed Drosophila

Probiotics are gaining much attention due to their beneficial functions in restoring microbial dysregulation which is the newly emerging etiology of the metabolic diseases such as hyperglycemia. The anti-hyperglycemic effects of probiotics were mostly demonstrated using rodents or human subjects, instead of in vitro cell lines due to the susceptibility to probiotics-derived acid compounds, leading to the ineligibility of in vitro cell lines for the massive screening of varied probiotic libraries. To address this issue, the current study was aimed to take advantage of the Drosophila to conduct the massive screening for the anti-hyperglycemic effects of 168 probiotic libraries belonging to 14 different species derived from human oral cavity. Each of 168 strains were individually treated to High-Sucrose Diet (HSD)-fed Drosophila and the lowest hemolymph glucose levels were measured in HSD-fed Drosophila supplemented with Lactiplantibacillus plantarum DM043, DM049, and DM083 among 168 strains. The Lpb. plantarum DM083 could survive at an acidic environment greater than DM043 and DM049. Heat-killed Lpb. plantarum DM083 failed to inhibit hyperglycemia, reflecting the importance of being alive. Importantly, Lpb. plantarum DM083-mediated molecular pathways related to insulin and glucose metabolisms were conserved from Drosophila to mammals, implying that Drosophila-tested Lpb. plantarum DM083 could be extrapolated to the rodents or humans. In conclusion, HSDfed Drosophila was for the first time applied to evaluate the anti-hyperglycemic effects of 168 probiotic libraries, leading to the discovery of Lpb. plantarum DM083 as the best candidate which will be applicable to the rodents and humans.