Stable Transfection of Plasmid DNA into Adherent Rodent Cell Lines Using Calcium Phosphate v1

This is the authors' abstract. We don't add key points for this paper.

A traditional strategy for stably transfecting DNA into rodent fibroblast cell lines features calcium phosphate precipitates.Here we describe our laboratory protocol for this strategy.We have assumed that the transfected DNA contains an expression vector for an antibiotic resistance gene, such as the neomycin resistance gene, the hygromycin resistance gene, the puromycin resistance gene, and the like.