The content and qualitative composition of starch in bread wheat samples

Starch is one of the main components of wheat endosperm and is used in the production of food and non-food products. The composition and content of starch largely determines quality of flour in breadmaking. The genotype and environmental conditions affect the quantity and qualitative composition of starch and the ratio of different types of starch granules in wheat endosperm. In this work, the qualitative and quantitative content of gluten, starch and starch fractions in the grain of wheat samples with hard and soft endosperm was studied to assess variability of traits. Wholemeal flour was separated into gluten, starch and dietary fiber by the "plant on the table" method. The isolated starch was separated into fractions A and B. Starch granules were examined using laser-scanning microscopy to determine their size. The analysis showed that the hard-grain variety Novosibirskaya 67 and the soft-grain variety Golubka could be the donors of high content of starch A. The hybrid lines SSL-2, SSL-2 and Rodina-M1 can be the donors of high content of starch B. For the studied samples, the proportion of small starch granules (<10 µm) was associated with grain endosperm structure.