Exploration of Key Regulatory Factors for Continuous Regulation of Osteogenic Differentiation in Mesenchymal Stem Cells Based on Transcriptomic Sequencing

Abstract In this study, we conducted an integrated analysis of high-throughput transcriptomic sequencing data to identify and validate the biological molecules that dynamically regulate the osteogenic differentiation of Mesenchymal Stem Cells (MSCs). Through transcriptomic sequencing analysis, taking into account the expression levels of genes in human tissues and experimental validation, we identified PTBP1, H2AFZ, and TTPAL (C20ORF121) as pivotal regulators that govern the fate of MSC osteogenic differentiation. This article represents the first utilization of high-throughput transcriptomics for identifying regulatory factors involved in MSC osteogenesis. This study holds significant importance in investigating crucial factors that determine the fate of MSC differentiation, with notable clinical implications. This approach will lead to a better understanding of the complex biological processes and molecular mechanisms underlying diseases.