A broad host phage, CP6, for combating multidrug-resistant Campylobacter prevalent in poultry meat

Campylobacter is a major cause of bacterial foodborne diarrhea worldwide. Consumption of raw or undercooked chicken meat contaminated with Campylobacter is the most common causative agent of human infections. Given the high prevalence of contamination in poultry meat and the recent rise of multi-drug-resistant (MDR) Campylobacter strains, an effective intervention method of reducing bird colonization or food-processing is needed. In this study, the Campylobacter-specific lytic phage CP6 was isolated from chicken feces. CP6 strongly infected MDR Campylobacter isolates from retail poultry meats. It had good pH (3–9) and temperature tolerance (<50 °C) and exhibited a broad host range of Campylobacter isolates (97.4%). The complete genome sequence revealed a linear double-stranded DNA (178,350 bp, group Ⅱ Campylobacter phage) with 27.51% GC content, including 209 predicted open reading frames, among which only 54 were annotated with known functions. Phylogenetic analysis of the phage major capsid protein demonstrated that phage CP6 was closely related to Campylobacter phage CPt10, CP21, CP20, IBB35, and CP220. CP6 phage exerted good antimicrobial effects on MDR Campylobacter in vitro culture and reduced CFUs of the host cells by up to 1-log compared with the control in artificially contaminated chicken breast meat. Our findings suggested the potential of CP6 phage as a promising antimicrobial agent for combating MDR Campylobacter in food processing.