Mesoporous Silica Nanomaterials Targeted Delivery of PDK Inhibitor and shRNA Dual Therapy for Osteosarcoma Inhibitory Activity and Mechanism

The purpose of this study is to construct a multi-drug delivery, dual anti-tumor, active targeting and drug control.The invention relates to a mesoporous silicon nano material (MSN) -nucleic acid nano drug delivery system with integrated release function, to explore its inhibitory activity and mechanism on osteosarcoma.Firstly, mesoporous silicon nanomaterials (MSN) were synthesized, and PDK inhibitors and shRNA were introduced into MSN.Then, aptamers were designed to achieve active targeting of tumor cells and controlled release of drugs.Next, through cellular uptake experiments and active targeting evaluation, the active targeting of the MSN-aptamer nanodrug system to tumor cells was determined.Through cell activity and proliferation inhibition experiments, apoptosis and cell cycle analysis, and cell migration and invasion ability analysis, the dual anti-tumor activity of drug system chemistry and gene therapy was determined.Finally, the antitumor effect and toxicity of the drug system were evaluated in animal experiments.The MSN-nucleic acid nanocarrier system was successfully constructed and characterized.Through active targeting evaluation, the active targeting of the MSN-aptamer nano-drug system to tumor cells was confirmed.The drug system shows anti-tumor activity by inhibiting cell proliferation, inducing cell apoptosis and cell cycle analysis.And the experiments of inhibiting cell migration and invasion ability prove the dual anti-tumor activity of the drug system.The results of animal experiments showed the anti-tumor effect of the drug system and evaluated its toxicity.The MSN-ribonucleic acid nano-drug delivery system has the potential to be used in anti-tumor therapy.The MSN-aptamer nano-drug system achieves the targeting effect on tumor cells through an active targeting mechanism.Multiple mechanisms may be involved in the dual antitumor activities of drug systems.The results of animal experiments show the anti-tumor effect of the drug system and its potential in clinical application.