Figure 5 from ESR1 F404 Mutations and Acquired Resistance to Fulvestrant in ESR1-Mutant Breast Cancer

Transcriptomic analysis of ESR1-mutant models. A, Gene set enrichment analysis (GSEA) for D538G + F404L models compared with D538G D6C cells maintained in 1 nmol/L estradiol. Pathways are highlighted red; false discovery rate-adjusted q value <0.05. B, GSEA for D538G + F404L models compared with D538G D6C cells treated with 1 μmol/L fulvestrant for 24 hours. Pathways are highlighted red; false discovery rate-adjusted q value <0.05. C, GSEA for ESR1-mutant models. Normalized enrichment score (NES) is shown for the indicated pathways. *, False discovery rate-adjusted q value <0.05. D, Heat map of “Estrogen response late” genes (log₂ expression) for ESR1-mutant models maintained in 1 nmol/L estradiol. E, Heat map of “Estrogen response late” genes (log₂ expression) for ESR1-mutant models treated with 1 μmol/L fulvestrant in the presence of 1 nmol/L estradiol.