Key result
Multiplexed iPSC screening identifies GATA4 and WAC inactivation as drivers of disrupted cardiac differentiation.
Population
16 iPSC lines including 10 WT, 2 GATA4 +/-, 2 GATA4 -/-, and 2 WAC +/-
Comparison
Genetically barcoded multiplex single cell… vs Wild-type (WT) iPSC lines
Design
Preclinical
Authors
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A novel genetically barcoded iPSC platform enables scalable and reliable functional assessment of variants of unknown significance in congenital heart disease.
A novel genetically barcoded iPSC platform enables scalable and reliable functional assessment of variants of unknown significance in congenital heart disease.
Xiao et al. (2025) studied Congenital heart disease (n=16). Genetically barcoded iPSC platform vs. Wild-type iPSC lines was evaluated on iPSC-CM cell state distributions and cardiac differentiation. A genetically barcoded multiplex platform using 16 iPSC lines successfully identified that GATA4 and WAC inactivation, along with a subset of CHD variants, altered cardiac differentiation.
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