Mg-1.6Gd Alloys Hot Rolled At Reduction Of 80% Through Cytotoxicity Test For Implant Material

The high number of traffic accidents in Indonesia causes many victims which suffer from broken bones. Treatment that can be conducted is bone implant installation. Additionally, because magnesium’s physical characteristics are nearly identical to those of human bone, it is a desirable material for biodegradable bone implants. However, in the human body, magnesium dissolves quickly before new tissue can develop. Consequently, in order to postpone the solubility of magnesium, it must be coupled with gadolinium. A cytotoxicity assay utilizing osteoblast cells was necessary prior to the Mg-Gd alloy being used as implant material. This study employed the MTT Assay technique to determine cell survival following treatment. Moreover, osteoblast cell-containing plates were filled with Mg-1.6Gd samples that had rolled with 80% at different temperatures of 400, 450, 500, and 550°C. The ELISA Plate Reader was used to read the MTT after the samples had been incubated for 3, 7, and 14 days. Since the average viability value is higher than 70%, the investigation demonstrates that the sample that rolled with an 80% reduction is non-toxic. Furthermore, samples that rolled with 80% at 400°C showed persistent viability values that are higher than 70%. This study aims to evaluate the cellular toxicity of Mg-1.6Gd alloy that has been processed with 80% hot rolling under various thermal conditions, in order to assess whether the alloy is toxic or non-toxic and therefore suitable for use as a base material for bone implants.