Results show nearly equal pollen fertility in Douglas-fir after one year in liquid nitrogen, suggesting effective storage methods.
Results indicated that Douglas-fir ( Pseudotsuga menziesii (Mirb.) Franco) pollen stored in liquid nitrogen (- 196°C) for 1 year was nearly as fertile as fresh pollen when used for controlled pollinations. Percent of filled seed was 79.9 for liquid nitrogen-stored pollen and 81.9 percent for fresh pollen of the current year. Filled seeds per cone averaged 46.3 and 47.9 for liquid nitrogen-stored pollen and fresh pollen, respectively. Flowers pollinated with fresh or stored pollen experienced significantly greater levels of flower abortion than flowers that were isolated but not pollinated; survival of flowers was 45.3, 64.5, and 93.8 percent, respectively. Methods used in cryogenic storage with liquid nitrogen are simple and enable tree improvement workers and forest geneticists to effectively store Douglas-fir pollen for at least 1 year without significant loss of fertility. Forest Sci. 31:569-574.
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Donald L. Copes (1985) studied this question.
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