This research examines hydrogen evolution activity in aerobic conditions using NiFe-hydrogenase from E. coli, highlighting its performance on TiO2 nanotubes.
ABSTRACT [FeFe]‐hydrogenase has been of great interest due to its high enzymatic activity for hydrogen evolution reactions (HERs). However, the big challenge of [FeFe]‐hydrogenase is a significant performance degradation in aerobic conditions. On the other hand, [NiFe]‐hydrogenase of E. coli has an oxygen tolerant property. Therefore, using [NiFe]‐hydrogenase is an effective solution to avoid performance degradation in aerobic conditions. Herein, we extracted [NiFe]‐hydrogenases from E. coli and immobilized them on the TiO 2 nanotube (TNT) electrode prepared by pyrrole‐based electropolymerization for application in aerobic conditions. As a result, we can confirm that [NiFe]‐hydrogenases coated TNT electrode demonstrates the increased HER activity underaerobic condition than control samples in in‐vitro activity test using methylene viologen and linear sweep voltammetry.
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Kim et al. (2025) studied this question.
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