ABSTRACT FeFe‐hydrogenase has been of great interest due to its high enzymatic activity for hydrogen evolution reactions (HERs). However, the big challenge of FeFe‐hydrogenase is a significant performance degradation in aerobic conditions. On the other hand, NiFe‐hydrogenase of E. coli has an oxygen tolerant property. Therefore, using NiFe‐hydrogenase is an effective solution to avoid performance degradation in aerobic conditions. Herein, we extracted NiFe‐hydrogenases from E. coli and immobilized them on the TiO 2 nanotube (TNT) electrode prepared by pyrrole‐based electropolymerization for application in aerobic conditions. As a result, we can confirm that NiFe‐hydrogenases coated TNT electrode demonstrates the increased HER activity underaerobic condition than control samples in in‐vitro activity test using methylene viologen and linear sweep voltammetry.
Kim et al. (Fri,) studied this question.