Rapid in situ encapsulation of NiFe-hydrogenase into covalent organic frameworks for robust hydrogen oxidation and evolution

Key Points

• The aim is to enhance the stability and efficiency of [NiFe] hydrogenases for hydrogen production by encapsulating them in covalent organic frameworks.
• Encapsulation of [NiFe] hydrogenase into covalent organic frameworks was performed in situ.
• The stability and activity of the encapsulated enzyme were tested under conditions mimicking biological systems.
• Hydrogen oxidation and evolution reactions were quantitatively analyzed.
• Encapsulated hydrogenases exhibited a 30% increase in activity compared to non-encapsulated controls.
• Stable performance was maintained over multiple reaction cycles, indicating improved durability.
• Hydrogen evolution rates reached 120 μmol H2/min under optimal conditions.